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human female fetal lung fibroblast imr90 cell line  (ATCC)


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    Structured Review

    ATCC human female fetal lung fibroblast imr90 cell line

    Human Female Fetal Lung Fibroblast Imr90 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imr90+female+human+fetal+lung+fibroblast+cells/IMR-90/pmc11530851-365-55-46
    Average 99 stars, based on 2172 article reviews
    human female fetal lung fibroblast imr90 cell line - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility"

    Article Title: Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility

    Journal: iScience

    doi: 10.1016/j.isci.2024.111096


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Protease Inhibitor, Reverse Transcription, Blocking Assay, Mass Spectrometry, SYBR Green Assay, Flow Cytometry, Imaging, Mutagenesis, Cell Cycle Assay, shRNA, Software

    Related Articles

    Expressing:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Quantitative RT-PCR:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    shRNA:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Transfection:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Infection:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Plasmid Preparation:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Control:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    BrdU Incorporation Assay:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Activity Assay:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Over Expression:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Positive Control:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Western Blot:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Staining:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Two Tailed Test:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Transduction:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Retroviral:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Knockdown:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Immunofluorescence:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    SDS Page:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Cell Culture:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Microscopy:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Stable Transfection:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Electroporation:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Transformation Assay:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Dispersion:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    High Content Screening:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Reverse Transcription:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Polymerase Chain Reaction:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Immunohistochemical staining:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Phospho-proteomics:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Biomarker Discovery:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Mutagenesis:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Activation Assay:

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines except THP-1 that were culture in RPMI-1640 Medium (Sigma), were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence
    Article Snippet: HEK293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).All cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma), supplemented with 10% Fetal Bovine Serum (FBS) (ThermoFisher) and 1% antibiotic-antimycotic solution (ThermoFisher).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype.
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).

    Article Title: The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Article Snippet: Human embryonic kidney (HEK) 293T and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific).



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    ATCC human female fetal lung fibroblast imr90 cell line

    Human Female Fetal Lung Fibroblast Imr90 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    imr90 female human fetal lung fibroblast cells  (ATCC)
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    Fig. 2 LPS-mediated caspase-4 induced senescence is independent of inflammasome priming. A CASP4, CASP1 or RASG12V were overexpressed in <t>IMR90</t> cells, IL1A and IL1B mRNA relative expression levels were quantified by RT-qPCR. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). B Cells were treated as shown in Fig. 1A. CASP1 or CASP4 expression was targeted by shRNA prior to LPS transfection with 0.1 μg LPS. IL1B mRNA relative expression was quantified by RT-qPCR 48 h after LPS transfection. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). C–E IMR90 cells were infected with CASP4 or RASG12V expression vectors or empty vector (vector) control. After 3 h treatment with Pam2CSK4, cells were transfected with 0.1 μg LPS. IL1B mRNA relative expression (C) and BrdU incorporation (D) were measured by IF and RT-qPCR respectively 48 h after LPS transfection. E SA-β-Galactosidase activity was determined 4 days after LPS transfection. Representative images for SA-β-Galactosidase activity are shown. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. Scale bar = 0.1 mm as indicated.
    Imr90 Female Human Fetal Lung Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imr90+female+human+fetal+lung+fibroblast+cells/IMR-90/pm34916628-263-8-18
    Average 99 stars, based on 1 article reviews
    imr90 female human fetal lung fibroblast cells - by Bioz Stars, 2026-09
    99/100 stars
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    Image Search Results


    Journal: iScience

    Article Title: Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility

    doi: 10.1016/j.isci.2024.111096

    Figure Lengend Snippet:

    Article Snippet: Stable clone of adult female human cervical adenocarcinoma HeLa cell line with long telomeres (HeLa1.3 cells); adult male human fibrosarcoma HT1080 cell line overexpressing telomase (HT1080 super-telomerase cells, HT1080-ST) (kindly provided by T. de Lange and J. Lingner, respectively); adult female human osteosarcoma U2OS cell line (ATCC); human embryonic kidney HEK293FT cell line (ATCC); and human female fetal lung fibroblast IMR90 cell line (Coriel Institute) were cultured in DMEM medium supplemented with 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich, F2442) and maintained at 37°C in 5% (v/v) CO 2 .

    Techniques: Virus, Recombinant, Protease Inhibitor, Reverse Transcription, Blocking Assay, Mass Spectrometry, SYBR Green Assay, Flow Cytometry, Imaging, Mutagenesis, Cell Cycle Assay, shRNA, Software

    Fig. 2 LPS-mediated caspase-4 induced senescence is independent of inflammasome priming. A CASP4, CASP1 or RASG12V were overexpressed in IMR90 cells, IL1A and IL1B mRNA relative expression levels were quantified by RT-qPCR. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). B Cells were treated as shown in Fig. 1A. CASP1 or CASP4 expression was targeted by shRNA prior to LPS transfection with 0.1 μg LPS. IL1B mRNA relative expression was quantified by RT-qPCR 48 h after LPS transfection. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). C–E IMR90 cells were infected with CASP4 or RASG12V expression vectors or empty vector (vector) control. After 3 h treatment with Pam2CSK4, cells were transfected with 0.1 μg LPS. IL1B mRNA relative expression (C) and BrdU incorporation (D) were measured by IF and RT-qPCR respectively 48 h after LPS transfection. E SA-β-Galactosidase activity was determined 4 days after LPS transfection. Representative images for SA-β-Galactosidase activity are shown. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. Scale bar = 0.1 mm as indicated.

    Journal: Cell death and differentiation

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.

    doi: 10.1038/s41418-021-00917-6

    Figure Lengend Snippet: Fig. 2 LPS-mediated caspase-4 induced senescence is independent of inflammasome priming. A CASP4, CASP1 or RASG12V were overexpressed in IMR90 cells, IL1A and IL1B mRNA relative expression levels were quantified by RT-qPCR. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). B Cells were treated as shown in Fig. 1A. CASP1 or CASP4 expression was targeted by shRNA prior to LPS transfection with 0.1 μg LPS. IL1B mRNA relative expression was quantified by RT-qPCR 48 h after LPS transfection. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). C–E IMR90 cells were infected with CASP4 or RASG12V expression vectors or empty vector (vector) control. After 3 h treatment with Pam2CSK4, cells were transfected with 0.1 μg LPS. IL1B mRNA relative expression (C) and BrdU incorporation (D) were measured by IF and RT-qPCR respectively 48 h after LPS transfection. E SA-β-Galactosidase activity was determined 4 days after LPS transfection. Representative images for SA-β-Galactosidase activity are shown. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. Scale bar = 0.1 mm as indicated.

    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.

    Techniques: Expressing, Quantitative RT-PCR, shRNA, Transfection, Infection, Plasmid Preparation, Control, BrdU Incorporation Assay, Activity Assay

    Fig. 3 Caspase-4 mediated regulation of senescence is independent of its catalytical function. A–C IMR90 cells were infected with wild- type (WT) CASP4, catalytically inactive (C258A) CASP4 or the empty vector (vector). Overexpression of RASG12V was used as a positive control for the induction of senescence. A Caspase-4 and IL-1β expression levels were investigated by immunoblotting. β-Actin immunoblot was performed for loading control. B Caspase-4 protein expression levels and BrdU incorporation measured by immunofluorescence, as well as SA-β-Galactosidase activity were assessed 4 days after equal number of cells were seeded. C Relative cell content (left) was quantified 15 days after equal number of cells were seeded; representative images (right) of crystal violet stained cells are shown. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). D–G (D) Schematic representation of the experiment shown in D-F and Supplementary Fig. 3G–H. IMR90 cells were infected with wild-type (WT) CASP4, catalytically inactive (C258A) CASP4 or the empty vector (vector) prior to transfection with 1 μg LPS. (E) Cell viability was measured 24 h after LPS transfection, (F) BrdU incorporation was measured by immunofluorescence 48 h after LPS transfection and (G) SA-β-Galactosidase activity was determined 4 days after LPS transfection. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. ns, not significant.

    Journal: Cell death and differentiation

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.

    doi: 10.1038/s41418-021-00917-6

    Figure Lengend Snippet: Fig. 3 Caspase-4 mediated regulation of senescence is independent of its catalytical function. A–C IMR90 cells were infected with wild- type (WT) CASP4, catalytically inactive (C258A) CASP4 or the empty vector (vector). Overexpression of RASG12V was used as a positive control for the induction of senescence. A Caspase-4 and IL-1β expression levels were investigated by immunoblotting. β-Actin immunoblot was performed for loading control. B Caspase-4 protein expression levels and BrdU incorporation measured by immunofluorescence, as well as SA-β-Galactosidase activity were assessed 4 days after equal number of cells were seeded. C Relative cell content (left) was quantified 15 days after equal number of cells were seeded; representative images (right) of crystal violet stained cells are shown. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). D–G (D) Schematic representation of the experiment shown in D-F and Supplementary Fig. 3G–H. IMR90 cells were infected with wild-type (WT) CASP4, catalytically inactive (C258A) CASP4 or the empty vector (vector) prior to transfection with 1 μg LPS. (E) Cell viability was measured 24 h after LPS transfection, (F) BrdU incorporation was measured by immunofluorescence 48 h after LPS transfection and (G) SA-β-Galactosidase activity was determined 4 days after LPS transfection. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05. ns, not significant.

    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.

    Techniques: Infection, Plasmid Preparation, Over Expression, Positive Control, Expressing, Western Blot, Control, BrdU Incorporation Assay, Activity Assay, Staining, Transfection, Two Tailed Test

    Fig. 4 The caspase-4 noncanonical inflammasome is activated in oncogene-induced senescence. A IMR90 cells were infected with RASG12V

    Journal: Cell death and differentiation

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.

    doi: 10.1038/s41418-021-00917-6

    Figure Lengend Snippet: Fig. 4 The caspase-4 noncanonical inflammasome is activated in oncogene-induced senescence. A IMR90 cells were infected with RASG12V

    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.

    Techniques: Infection

    Fig. 6 Caspase-4 contributes to the arrest in cell proliferation in OIS. A IMR90 ER:STOP and ER:RAS cells were transfected with control (NTP), two individual CASP4-targeting siRNAs (CASP4-1 and CASP4-2) or a pool of 4 different siRNA sequences targeting CASP4 (CASP4-p), and treated with 4OHT or not as indicated. BrdU incorporation was measured by immunofluorescence 5 days after 4OHT addition. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. B, C IMR90 cells were transduced with an empty retroviral vector (vector) or shRNAs targeting either CASP4 (shCASP4) or TP53 (shP53). B Lysates were subjected to immunoblotting analyses with the indicated antibodies eight days after 4OHT addition. C On day 0, equal number of cells were subjected to 4OHT treatment. Fifteen days after 4OHT addition, plates were fixed and stained with crystal violet. Crystal violet was extracted and used to quantify cell content. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 4 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. D Related to Fig. 5A. DEG analysis was performed between control ER:STOP and CASP4-knockdown ER:RAS compared to control ER:RAS after 5 days of 4OHT treatment. Heatmap of the log2FC values from the indicated genes. E IMR90 ER:STOP and ER:RAS cells were transfected with control (NTP), CASP1 or CASP4- targeting siRNAs and treated with 4OHT during 4 days. Cell lysates were subjected to immunoblotting analyses with the indicated antibodies. F IMR90 ER:RAS cells were transfected with control (NTP), CASP1 or CASP4-targeting siRNA and mRNA relative expression of the indicated genes was quantified by RT-qPCR after 5 days of 4OHT treatment. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). ***P < 0.001, **P < 0.01, *P < 0.05 and ns, not significant.

    Journal: Cell death and differentiation

    Article Title: Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence.

    doi: 10.1038/s41418-021-00917-6

    Figure Lengend Snippet: Fig. 6 Caspase-4 contributes to the arrest in cell proliferation in OIS. A IMR90 ER:STOP and ER:RAS cells were transfected with control (NTP), two individual CASP4-targeting siRNAs (CASP4-1 and CASP4-2) or a pool of 4 different siRNA sequences targeting CASP4 (CASP4-p), and treated with 4OHT or not as indicated. BrdU incorporation was measured by immunofluorescence 5 days after 4OHT addition. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. B, C IMR90 cells were transduced with an empty retroviral vector (vector) or shRNAs targeting either CASP4 (shCASP4) or TP53 (shP53). B Lysates were subjected to immunoblotting analyses with the indicated antibodies eight days after 4OHT addition. C On day 0, equal number of cells were subjected to 4OHT treatment. Fifteen days after 4OHT addition, plates were fixed and stained with crystal violet. Crystal violet was extracted and used to quantify cell content. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 4 independent experiments. Statistical analysis was performed using two-tailed Student’s t test. D Related to Fig. 5A. DEG analysis was performed between control ER:STOP and CASP4-knockdown ER:RAS compared to control ER:RAS after 5 days of 4OHT treatment. Heatmap of the log2FC values from the indicated genes. E IMR90 ER:STOP and ER:RAS cells were transfected with control (NTP), CASP1 or CASP4- targeting siRNAs and treated with 4OHT during 4 days. Cell lysates were subjected to immunoblotting analyses with the indicated antibodies. F IMR90 ER:RAS cells were transfected with control (NTP), CASP1 or CASP4-targeting siRNA and mRNA relative expression of the indicated genes was quantified by RT-qPCR after 5 days of 4OHT treatment. Graph bars, error bars and dots represent respectively the mean ± s.e.m. and the individual values of 3 independent experiments. Statistical analysis was performed using one-way analysis of variance (ANOVA). ***P < 0.001, **P < 0.01, *P < 0.05 and ns, not significant.

    Article Snippet: HEK293T, MEFs, CAPAN-1, PSN-1, A549, HCT116, THP-1, and IMR90 female human fetal lung fibroblast cells were obtained from American Type Culture Collection.

    Techniques: Transfection, Control, BrdU Incorporation Assay, Two Tailed Test, Transduction, Retroviral, Plasmid Preparation, Western Blot, Staining, Knockdown, Expressing, Quantitative RT-PCR